In this paper, published in the PDA Journal, the M3 collaboration explore the challenges presented by the Bio-Fluorescent Particle Counting (BFPC) technology, when considering that the traditional Colony Forming Unit (CFU) is the gold standard which any change is measured against. We examine why attempts to correlate newer units of measure used by Bio-Fluorescent Particle Counters, namely the Auto-Fluorescent Units (AFUs), to the traditional CFUs are not necessarily appropriate. The paper explores in depth why there is no consistent correlation factor between the two units of measure, and why that should not be a barrier to fully leveraging, implementing, and using such modern technologies in routine monitoring.
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