Challenges encountered in the implementation of bio-fluorescent particle counting systems as a routine microbial monitoring tool

The transition from traditional growth-based microbial detection methods to continuous bio-fluorescent particle counting methods represents a paradigm shift, because the results will be non-equivalent in terms of microbial counts, and a continuous, rather than periodic, data stream will be available. Bio-fluorescent particle counting technology, a type of rapid microbiological method, uses the detection of intrinsic fluorescence of microbial cells to enumerate bioburden levels in air or water samples, continuously. The reported unit is commonly referred to as an auto-fluorescence unit, which is not dependent upon growth, as is the traditional method. This article published in the PDA Journal of Pharmaceutical Science and Technology discusses challenges encountered when implementing this modern technology, and the perspective from a consortium of four industry working groups including BioPhorum on navigating these challenges.