Host cell protein searchable database

Please Note

This database is no longer maintained or updated.

If you want to access the latest HCP database or learn more about BioPhorum please contact hello@biophorum.com

Host cell proteins (HCPs) are process-related impurities that may co-purify with biopharmaceutical drug products. Within this class of impurities there are some that are more problematic. These problematic HCPs can be considered high-risk and can include those that are immunogenic, biologically active, or enzymatically active with the potential to degrade either product molecules or excipients used in formulation.

The “High‐risk” host cell proteins (HCPs): A multi‐company collaborative view publication and database produced by the host cell protein workstream provide a comprehensive review/list of potential problematic HCPs that could impact the safety, efficacy, and quality aspects of CHO-produced biologics, during their development and manufacturing. It is a great reference on the best practice and control strategy for “high-risk” HCPs” in the biopharmaceutical industry.

The database currently focuses on the HCPs specifically isolated from biotherapeutics produced in CHO cells, which are frequently seen HCPs found throughout different processing steps. These HCPs were identified by the BioPhorum’s development group’s Host Cell Protein workstream, through literature review and industry survey data.

The key purpose of the database is also to provide information on HCPs that are considered to be problematic and therefore “high-risk.” It further classifies them into four major categories based on their impact to:

  • Product quality
  • Formulation
  • Direct biological function in humans
  • Immunogenicity.

Ultimately, the team hopes to include information on the function of all the listed proteins, the specific type of impact (drug or patient) such as aggregation, fragmentation, modification or immunogenicity and the associated references for each protein. Currently this information is only listed for the “high risk” HCPs within the database (rows are highlighted in RED and this list may be filtered by clicking the high risk HCP box in the search field below).

 


Identified HCP (Abbreviation) High risk?Mw (kDa) pI UniProt Accession No. No. of amino acids
60S acidic ribosomal protein P0 No30.68.9G3HKG9280 Read more
Identified HCP (Abbreviation)60S acidic ribosomal protein P0
High risk HCPNo
MW (kDa)30.6
pI8.9
UniProt Accession No.G3HKG9
No. of amino acids280
40S ribosomal protein SA (RPSA) No19.79.4G3HQX0179 Read more
Identified HCP (Abbreviation)40S ribosomal protein SA (RPSA)
High risk HCPNo
MW (kDa)19.7
pI9.4
UniProt Accession No.G3HQX0
No. of amino acids179
Cathepsin Z (CatZ) High Risk347.5Q9EPP7306 Read more
Identified HCP (Abbreviation)Cathepsin Z (CatZ)
High risk HCPYess
MW (kDa)34
pI7.5
UniProt Accession No.Q9EPP7
No. of amino acids306
Function
Impact
Type of impact
StatusBrix et al., 2018; Chiverton et al., 2016; Park et al., 2017
Cathepsin L (CatL) High Risk37.36.8G3INC5333 Read more
Identified HCP (Abbreviation)Cathepsin L (CatL)
High risk HCPYess
MW (kDa)37.3
pI6.8
UniProt Accession No.G3INC5
No. of amino acids333
Function
Impact
Type of impact
StatusGao et al., 2011; Luo et al., 2018; Park et al., 2017
Cathepsin E (CatE) No42.24.7G3HAY9388 Read more
Identified HCP (Abbreviation)Cathepsin E (CatE)
High risk HCPNo
MW (kDa)42.2
pI4.7
UniProt Accession No.G3HAY9
No. of amino acids388
Cathepsin D (CatD) No44.16.5G3I4W7408 Read more
Identified HCP (Abbreviation)Cathepsin D (CatD)
High risk HCPNo
MW (kDa)44.1
pI6.5
UniProt Accession No.G3I4W7
No. of amino acids408
Cathepsin B (CatB) High Risk37.55.7G3H0L9339 Read more
Identified HCP (Abbreviation)Cathepsin B (CatB)
High risk HCPYess
MW (kDa)37.5
pI5.7
UniProt Accession No.G3H0L9
No. of amino acids339
Function
Impact
Type of impact
StatusAboulaich et al., 2014; Albrecht et al., 2018b; Levy et al., 2016; Migani et al., 2017; Gao et al., 2011; Yang et al., 2019; Zhang et al., 2016; Zhang et al., 2019
Catalase No63.18.3G3GYY6555 Read more
Identified HCP (Abbreviation)Catalase
High risk HCPNo
MW (kDa)63.1
pI8.3
UniProt Accession No.G3GYY6
No. of amino acids555
Carboxypeptidase D (Cpd) High Risk123.45.8G3HR951106 Read more
Identified HCP (Abbreviation)Carboxypeptidase D (Cpd)
High risk HCPYess
MW (kDa)123.4
pI5.8
UniProt Accession No.G3HR95
No. of amino acids1106
Function
Impact
Type of impact
StatusHu et al., 2016; Dick et al., 2008 (Dick, Qiu, Mahon, Adamo, & Cheng, 2008)
Carboxylesterase B-1-like protein, Liver (CES-B1L) No77.97.9A0A061I7X9704 Read more
Identified HCP (Abbreviation)Carboxylesterase B-1-like protein, Liver (CES-B1L)
High risk HCPNo
MW (kDa)77.9
pI7.9
UniProt Accession No.A0A061I7X9
No. of amino acids704
Carboxylesterase 1-like protein, Liver (CES1) High Risk97.55.9A0A061IFE2882 Read more
Identified HCP (Abbreviation)Carboxylesterase 1-like protein, Liver (CES1)
High risk HCPYess
MW (kDa)97.5
pI5.9
UniProt Accession No.A0A061IFE2
No. of amino acids882
Function
Impact
Type of impact
StatusZhang et al., 2020
Calmodulin (CaM) No16.74.1A0A061HUH1149 Read more
Identified HCP (Abbreviation)Calmodulin (CaM)
High risk HCPNo
MW (kDa)16.7
pI4.1
UniProt Accession No.A0A061HUH1
No. of amino acids149
C-X-C motif chemokine 3 (CXCL3) High Risk119.1A4URF0101 Read more
Identified HCP (Abbreviation)C-X-C motif chemokine 3 (CXCL3)
High risk HCPYess
MW (kDa)11
pI9.1
UniProt Accession No.A4URF0
No. of amino acids101
Function
Impact
Type of impact
StatusFarrell et al., 2015; Gilgunn & Bones, 2018; Zhang et al., 2014
C-C motif chemokine No15.99.3G3GTT2143 Read more
Identified HCP (Abbreviation)C-C motif chemokine
High risk HCPNo
MW (kDa)15.9
pI9.3
UniProt Accession No.G3GTT2
No. of amino acids143
Annexin A5 No36.14.9G3I5A4321 Read more
Identified HCP (Abbreviation)Annexin A5
High risk HCPNo
MW (kDa)36.1
pI4.9
UniProt Accession No.G3I5A4
No. of amino acids321
Annexin A2 No275.7G3IG05244 Read more
Identified HCP (Abbreviation)Annexin A2
High risk HCPNo
MW (kDa)27
pI5.7
UniProt Accession No.G3IG05
No. of amino acids244
Alpha-enolase (2-phospho-D-glycerate hydro-lyase) High Risk15.55G3I0W1139 Read more
Identified HCP (Abbreviation)Alpha-enolase (2-phospho-D-glycerate hydro-lyase)
High risk HCPYess
MW (kDa)15.5
pI5
UniProt Accession No.G3I0W1
No. of amino acids139
Function
Impact
Type of impact
StatusLiu et al., 2019
Actin, cytoplasmic 1 (ACTB) No41.75.2P48975375 Read more
Identified HCP (Abbreviation)Actin, cytoplasmic 1 (ACTB)
High risk HCPNo
MW (kDa)41.7
pI5.2
UniProt Accession No.P48975
No. of amino acids375
78 kDa glucose regulated protein(GRP78, BiP) High Risk72.45.1G3I8R9654 Read more
Identified HCP (Abbreviation)78 kDa glucose regulated protein(GRP78, BiP)
High risk HCPYess
MW (kDa)72.4
pI5.1
UniProt Accession No.G3I8R9
No. of amino acids654
Function
Impact
Type of impact
StatusAboulaich et al., 2014; Albrecht et al., 2018b; Chiverton et al., 2016; Falkenberg et al., 2019; Kreimer et al., 2017; Liu et al., 2019; Migani et al., 2017; Zhang et al., 2014; Joucla et al., 2013; Levy et al., 2016
Calreticulin (CALR) No48.44.3G3HCX8417 Read more
Identified HCP (Abbreviation)Calreticulin (CALR)
High risk HCPNo
MW (kDa)48.4
pI4.3
UniProt Accession No.G3HCX8
No. of amino acids417
Galectin-3 (Gal-3) No32.46.9G3H7B3281 Read more
Identified HCP (Abbreviation)Galectin-3 (Gal-3)
High risk HCPNo
MW (kDa)32.4
pI6.9
UniProt Accession No.G3H7B3
No. of amino acids281
Glutathione S-transferase P 1 (GSTP1) High Risk258.2G3I3Y6221 Read more
Identified HCP (Abbreviation)Glutathione S-transferase P 1 (GSTP1)
High risk HCPYess
MW (kDa)25
pI8.2
UniProt Accession No.G3I3Y6
No. of amino acids221
Function
Impact
Type of impact
StatusAboulaich et al., 2014; Gilgunn & Bones, 2018; Zhang et al., 2014; Albrecht et al., 2018b
Glyceraldehyde-3-phosphate dehydrogenase (G3P) No35.78.5P17244333 Read more
Identified HCP (Abbreviation)Glyceraldehyde-3-phosphate dehydrogenase (G3P)
High risk HCPNo
MW (kDa)35.7
pI8.5
UniProt Accession No.P17244
No. of amino acids333
Granulins (GRN) No63.96G3HLK3592 Read more
Identified HCP (Abbreviation)Granulins (GRN)
High risk HCPNo
MW (kDa)63.9
pI6
UniProt Accession No.G3HLK3
No. of amino acids592
GTP-binding nuclear protein (RAN) No24.58.4G3IHE5216 Read more
Identified HCP (Abbreviation)GTP-binding nuclear protein (RAN)
High risk HCPNo
MW (kDa)24.5
pI8.4
UniProt Accession No.G3IHE5
No. of amino acids216
Guanine nucleotide-binding protein beta-2-like 1 (RACK1) No30.47G3HK00276 Read more
Identified HCP (Abbreviation)Guanine nucleotide-binding protein beta-2-like 1 (RACK1)
High risk HCPNo
MW (kDa)30.4
pI7
UniProt Accession No.G3HK00
No. of amino acids276
Heat shock cognate 71 kDa protein (HSPA8) No70.85.2P19378646 Read more
Identified HCP (Abbreviation)Heat shock cognate 71 kDa protein (HSPA8)
High risk HCPNo
MW (kDa)70.8
pI5.2
UniProt Accession No.P19378
No. of amino acids646
Heat shock protein HSP 90-alpha (HSP90A) No84.85P46633733 Read more
Identified HCP (Abbreviation)Heat shock protein HSP 90-alpha (HSP90A)
High risk HCPNo
MW (kDa)84.8
pI5
UniProt Accession No.P46633
No. of amino acids733
Heat shock protein HSP 90-beta (HSP90B) No47.85.3G3HC84412 Read more
Identified HCP (Abbreviation)Heat shock protein HSP 90-beta (HSP90B)
High risk HCPNo
MW (kDa)47.8
pI5.3
UniProt Accession No.G3HC84
No. of amino acids412
Endoplasmin (HSP90B1) No92.64.7G3HQM6803 Read more
Identified HCP (Abbreviation)Endoplasmin (HSP90B1)
High risk HCPNo
MW (kDa)92.6
pI4.7
UniProt Accession No.G3HQM6
No. of amino acids803
Galectin 3 binding protein (Gal-3BP) No63.85.1G3H3E4574 Read more
Identified HCP (Abbreviation)Galectin 3 binding protein (Gal-3BP)
High risk HCPNo
MW (kDa)63.8
pI5.1
UniProt Accession No.G3H3E4
No. of amino acids574
Fructose-bisphosphate aldolase (FBA) No39.48.3G3I4H6364 Read more
Identified HCP (Abbreviation)Fructose-bisphosphate aldolase (FBA)
High risk HCPNo
MW (kDa)39.4
pI8.3
UniProt Accession No.G3I4H6
No. of amino acids364
Follistatin-related protein 1 (FST1) No65.46.2G3HAI3583 Read more
Identified HCP (Abbreviation)Follistatin-related protein 1 (FST1)
High risk HCPNo
MW (kDa)65.4
pI6.2
UniProt Accession No.G3HAI3
No. of amino acids583
ERP57 protein No56.86Q91Z81505 Read more
Identified HCP (Abbreviation)ERP57 protein
High risk HCPNo
MW (kDa)56.8
pI6
UniProt Accession No.Q91Z81
No. of amino acids505
Galectin-1 (Gal-1) No14.85.5P48538135 Read more
Identified HCP (Abbreviation)Galectin-1 (Gal-1)
High risk HCPNo
MW (kDa)14.8
pI5.5
UniProt Accession No.P48538
No. of amino acids135
Elongation factor 2 (eEF2) No95.36.2P09445858 Read more
Identified HCP (Abbreviation)Elongation factor 2 (eEF2)
High risk HCPNo
MW (kDa)95.3
pI6.2
UniProt Accession No.P09445
No. of amino acids858
Elongation factor 1-alpha (eEF1a) No50.19.1P62629462 Read more
Identified HCP (Abbreviation)Elongation factor 1-alpha (eEF1a)
High risk HCPNo
MW (kDa)50.1
pI9.1
UniProt Accession No.P62629
No. of amino acids462
Cofilin-1 (CFL) No18.58.2G3IDM2166 Read more
Identified HCP (Abbreviation)Cofilin-1 (CFL)
High risk HCPNo
MW (kDa)18.5
pI8.2
UniProt Accession No.G3IDM2
No. of amino acids166
Clusterin (CLU) High Risk51.85.5G3HNJ3447 Read more
Identified HCP (Abbreviation)Clusterin (CLU)
High risk HCPYess
MW (kDa)51.8
pI5.5
UniProt Accession No.G3HNJ3
No. of amino acids447
Function
Impact
Type of impact
StatusAboulaich et al., 2014; Albrecht et al., 2018a; Farrell et al., 2015; Kreimer et al., 2017; Levy et al., 2016; Migani et al., 2017; Singh et al., 2020; Zhang et al., 2014; Doneanu et al., 2012; Levy et al., 2014; Wilson & Easterbrook-Smith, 1992
Chondroitin sulfate proteoglycan 4 (CSPG4) No2525.4G3H0E42323 Read more
Identified HCP (Abbreviation)Chondroitin sulfate proteoglycan 4 (CSPG4)
High risk HCPNo
MW (kDa)252
pI5.4
UniProt Accession No.G3H0E4
No. of amino acids2323
Lysosomal Acid Lipase (LAL) High Risk45.67.3G3HQY6397 Read more
Identified HCP (Abbreviation)Lysosomal Acid Lipase (LAL)
High risk HCPYess
MW (kDa)45.6
pI7.3
UniProt Accession No.G3HQY6
No. of amino acids397
Function
Impact
Type of impact
StatusLevy et al., 2014; McShan et al., 2016; Kreimer et al., 2017; Valente et al., 2015
Lysosomal Phospholipase A2 (LPLA2) No47.26.1G3HKV9412 Read more
Identified HCP (Abbreviation)Lysosomal Phospholipase A2 (LPLA2)
High risk HCPNo
MW (kDa)47.2
pI6.1
UniProt Accession No.G3HKV9
No. of amino acids412
Lysosomal protective protein (CTSA) No54.25.7G3H8V5475 Read more
Identified HCP (Abbreviation)Lysosomal protective protein (CTSA)
High risk HCPNo
MW (kDa)54.2
pI5.7
UniProt Accession No.G3H8V5
No. of amino acids475
Malate dehydrogenase, cytoplasmic (MDHC) No36.56.2G3HDQ2334 Read more
Identified HCP (Abbreviation)Malate dehydrogenase, cytoplasmic (MDHC)
High risk HCPNo
MW (kDa)36.5
pI6.2
UniProt Accession No.G3HDQ2
No. of amino acids334
Matrix metalloproteinase-19 (MMP-19) High Risk58.97.7G3HRK9525 Read more
Identified HCP (Abbreviation)Matrix metalloproteinase-19 (MMP-19)
High risk HCPYess
MW (kDa)58.9
pI7.7
UniProt Accession No.G3HRK9
No. of amino acids525
Function
Impact
Type of impact
StatusAboulaich et al., 2014; Farrell et al., 2015; Gilgunn & Bones, 2018; Levy et al., 2016;
Metalloproteinase inhibitor 1 (TIMP1) No22.48.8G3IBH0203 Read more
Identified HCP (Abbreviation)Metalloproteinase inhibitor 1 (TIMP1)
High risk HCPNo
MW (kDa)22.4
pI8.8
UniProt Accession No.G3IBH0
No. of amino acids203
Monocyte Chemoattractant Protein-1 (MCP-1) High Risk15.99.3G3GTT2143 Read more
Identified HCP (Abbreviation)Monocyte Chemoattractant Protein-1 (MCP-1)
High risk HCPYess
MW (kDa)15.9
pI9.3
UniProt Accession No.G3GTT2
No. of amino acids143
Function
Impact
Type of impact
StatusLeister et al., 2019; Yoshimura & Leonard, 1989
Myosin-9 (MYH9) No225.85.5G3IH631948 Read more
Identified HCP (Abbreviation)Myosin-9 (MYH9)
High risk HCPNo
MW (kDa)225.8
pI5.5
UniProt Accession No.G3IH63
No. of amino acids1948
Nidogen-1 (NID1) No30.18.4G3I3U5278 Read more
Identified HCP (Abbreviation)Nidogen-1 (NID1)
High risk HCPNo
MW (kDa)30.1
pI8.4
UniProt Accession No.G3I3U5
No. of amino acids278
Inter-alpha-trypsin inhibitor heavy chain H5 isoform X2 (ITIH5L) No107.98.5G3H7S9983 Read more
Identified HCP (Abbreviation)Inter-alpha-trypsin inhibitor heavy chain H5 isoform X2 (ITIH5L)
High risk HCPNo
MW (kDa)107.9
pI8.5
UniProt Accession No.G3H7S9
No. of amino acids983
Legumain (LGMN) No49.66.1G3I1H5438 Read more
Identified HCP (Abbreviation)Legumain (LGMN)
High risk HCPNo
MW (kDa)49.6
pI6.1
UniProt Accession No.G3I1H5
No. of amino acids438
Laminin subunit gamma-1 (LAMC1) No172.15G3HG251559 Read more
Identified HCP (Abbreviation)Laminin subunit gamma-1 (LAMC1)
High risk HCPNo
MW (kDa)172.1
pI5
UniProt Accession No.G3HG25
No. of amino acids1559
Laminin subunit beta-1 (LAMB1) No178.14.8G3I2781617 Read more
Identified HCP (Abbreviation)Laminin subunit beta-1 (LAMB1)
High risk HCPNo
MW (kDa)178.1
pI4.8
UniProt Accession No.G3I278
No. of amino acids1617
lactotransferrin (LTF) No71.48.6G3HTA2648 Read more
Identified HCP (Abbreviation)lactotransferrin (LTF)
High risk HCPNo
MW (kDa)71.4
pI8.6
UniProt Accession No.G3HTA2
No. of amino acids648
Lactadherin (MFG-E8) No16.19.5G3ICD3140 Read more
Identified HCP (Abbreviation)Lactadherin (MFG-E8)
High risk HCPNo
MW (kDa)16.1
pI9.5
UniProt Accession No.G3ICD3
No. of amino acids140
L-lactate dehydrogenase A chain (LDHA) No36.57Q06BU8332 Read more
Identified HCP (Abbreviation)L-lactate dehydrogenase A chain (LDHA)
High risk HCPNo
MW (kDa)36.5
pI7
UniProt Accession No.Q06BU8
No. of amino acids332
Histone H2B Type 1-N (H2B1N) No13.910.3G3HDU3126 Read more
Identified HCP (Abbreviation)Histone H2B Type 1-N (H2B1N)
High risk HCPNo
MW (kDa)13.9
pI10.3
UniProt Accession No.G3HDU3
No. of amino acids126
Histone H2A No26.710.9G3HDS3236 Read more
Identified HCP (Abbreviation)Histone H2A
High risk HCPNo
MW (kDa)26.7
pI10.9
UniProt Accession No.G3HDS3
No. of amino acids236
Hexosaminidase, beta No60.76G3H3P528 Read more
Identified HCP (Abbreviation)Hexosaminidase, beta
High risk HCPNo
MW (kDa)60.7
pI6
UniProt Accession No.G3H3P
No. of amino acids528
Lipoprotein Lipase (LPL) High Risk50.58G3H6V7450 Read more
Identified HCP (Abbreviation)Lipoprotein Lipase (LPL)
High risk HCPYess
MW (kDa)50.5
pI8
UniProt Accession No.G3H6V7
No. of amino acids450
Function
Impact
Type of impact
StatusChiu et al., 2017; Levy et al., 2014; McShan et al., 2016; Levy et al., 2016; Singh et al., 2020
Procollagen-lysine 2-oxoglutarate 5-deoxygenase_1 (PLOD1) High Risk765.8G3IIE7659 Read more
Identified HCP (Abbreviation)Procollagen-lysine 2-oxoglutarate 5-deoxygenase_1 (PLOD1)
High risk HCPYess
MW (kDa)76
pI5.8
UniProt Accession No.G3IIE7
No. of amino acids659
Function
Impact
Type of impact
StatusHogwood et al., 2016; Jawa et al., 2016
Phospholipid transfer protein (PLTP) No54.46.2G3H8V4493 Read more
Identified HCP (Abbreviation)Phospholipid transfer protein (PLTP)
High risk HCPNo
MW (kDa)54.4
pI6.2
UniProt Accession No.G3H8V4
No. of amino acids493
Proteasome subunit alpha type-7 (PSA7) No23.88.6G3GWR8212 Read more
Identified HCP (Abbreviation)Proteasome subunit alpha type-7 (PSA7)
High risk HCPNo
MW (kDa)23.8
pI8.6
UniProt Accession No.G3GWR8
No. of amino acids212
Protein S100-A6 (S10A6) High Risk105.3G3HC3189 Read more
Identified HCP (Abbreviation)Protein S100-A6 (S10A6)
High risk HCPYess
MW (kDa)10
pI5.3
UniProt Accession No.G3HC31
No. of amino acids89
Function
Impact
Type of impact
StatusAboulaich et al., 2014; Gilgunn & Bones, 2018; Paintlia et al., 2004
Pyruvate Kinase (PK) High Risk51.67.6G3H3Q1472 Read more
Identified HCP (Abbreviation)Pyruvate Kinase (PK)
High risk HCPYess
MW (kDa)51.6
pI7.6
UniProt Accession No.G3H3Q1
No. of amino acids472
Function
Impact
Type of impact
StatusChiverton et al., 2016; Goey et al., 2018; Zhang et al., 2014
Serine protease (HTRA1) High Risk28.76.5G3IBF4268 Read more
Identified HCP (Abbreviation)Serine protease (HTRA1)
High risk HCPYess
MW (kDa)28.7
pI6.5
UniProt Accession No.G3IBF4
No. of amino acids268
Function
Impact
Type of impact
StatusAboulaich et al., 2014; Dorai et al., 2011; Falkenberg et al., 2019; Gilgunn & Bones, 2018; Goey et al., 2018; Zhang et al., 2014
Sialate o-acetylesterase (SIAE) High Risk59.38.6A0A3L7HS03529 Read more
Identified HCP (Abbreviation)Sialate o-acetylesterase (SIAE)
High risk HCPYess
MW (kDa)59.3
pI8.6
UniProt Accession No.A0A3L7HS03
No. of amino acids529
Function
Impact
Type of impact
Status
Sulfated glycoprotein (SGP-1) No27.45.4G3I1Y9249 Read more
Identified HCP (Abbreviation)Sulfated glycoprotein (SGP-1)
High risk HCPNo
MW (kDa)27.4
pI5.4
UniProt Accession No.G3I1Y9
No. of amino acids249
Transforming Growth Factor-b1 (TGF-b1) High Risk26.48.5G3IA12237 Read more
Identified HCP (Abbreviation)Transforming Growth Factor-b1 (TGF-b1)
High risk HCPYess
MW (kDa)26.4
pI8.5
UniProt Accession No.G3IA12
No. of amino acids237
Function
Impact
Type of impact
StatusBeatson et al., 2011; Vanderlaan et al., 2018
Transgelin-2 (TAGLN) No22.58.8G3H7Z2199 Read more
Identified HCP (Abbreviation)Transgelin-2 (TAGLN)
High risk HCPNo
MW (kDa)22.5
pI8.8
UniProt Accession No.G3H7Z2
No. of amino acids199
Procollagen C-endopeptidase enhancer (PCPE1) No55.28.5G3I664509 Read more
Identified HCP (Abbreviation)Procollagen C-endopeptidase enhancer (PCPE1)
High risk HCPNo
MW (kDa)55.2
pI8.5
UniProt Accession No.G3I664
No. of amino acids509
Plectin-1 (Plec1) No83.48.7G3HWJ2720 Read more
Identified HCP (Abbreviation)Plectin-1 (Plec1)
High risk HCPNo
MW (kDa)83.4
pI8.7
UniProt Accession No.G3HWJ2
No. of amino acids720
Phospholipase D3 (PLD3) No54.46G3HNQ5488 Read more
Identified HCP (Abbreviation)Phospholipase D3 (PLD3)
High risk HCPNo
MW (kDa)54.4
pI6
UniProt Accession No.G3HNQ5
No. of amino acids488
Phospholipase B-like 2 (PLBL2) High Risk65.55.9G3I6T1585 Read more
Identified HCP (Abbreviation)Phospholipase B-like 2 (PLBL2)
High risk HCPYess
MW (kDa)65.5
pI5.9
UniProt Accession No.G3I6T1
No. of amino acids585
Function
Impact
Type of impact
StatusAboulaich et al., 2014; de Zafra et al., 2015; Fischer et al., 2017; Jawa et al., 2016; Migani et al., 2017
Phosphoglycerate mutase 1 (Pgam1) No20.27.8G3GZW8178 Read more
Identified HCP (Abbreviation)Phosphoglycerate mutase 1 (Pgam1)
High risk HCPNo
MW (kDa)20.2
pI7.8
UniProt Accession No.G3GZW8
No. of amino acids178
Phosphoglycerate kinase (PGK1) No44.68P50310417 Read more
Identified HCP (Abbreviation)Phosphoglycerate kinase (PGK1)
High risk HCPNo
MW (kDa)44.6
pI8
UniProt Accession No.P50310
No. of amino acids417
Peroxiredoxin-1 (PRDX1) High Risk22.38.2Q9JKY1199 Read more
Identified HCP (Abbreviation)Peroxiredoxin-1 (PRDX1)
High risk HCPYess
MW (kDa)22.3
pI8.2
UniProt Accession No.Q9JKY1
No. of amino acids199
Function
Impact
Type of impact
StatusAboulaich et al., 2014; Albrecht et al., 2018b; Chiverton et al., 2016; Falkenberg et al., 2019; Jawa et al., 2016; Kreimer et al., 2017; Liu et al., 2019; Migani et al., 2017, Zhang et al., 2014
Peptidyl-prolyl cis-trans isomerase A (PPIA) No17.98.4P14851164 Read more
Identified HCP (Abbreviation)Peptidyl-prolyl cis-trans isomerase A (PPIA)
High risk HCPNo
MW (kDa)17.9
pI8.4
UniProt Accession No.P14851
No. of amino acids164
Nucleoside diphosphate kinase B (NDPK-B) No17.37.8G3HBD3152 Read more
Identified HCP (Abbreviation)Nucleoside diphosphate kinase B (NDPK-B)
High risk HCPNo
MW (kDa)17.3
pI7.8
UniProt Accession No.G3HBD3
No. of amino acids152
Nucleobindin-2 (NUCB2) No50.35.1G3IF52420 Read more
Identified HCP (Abbreviation)Nucleobindin-2 (NUCB2)
High risk HCPNo
MW (kDa)50.3
pI5.1
UniProt Accession No.G3IF52
No. of amino acids420
Transketolase (TKTase) No67.77.6G3GUU5 623 Read more
Identified HCP (Abbreviation)Transketolase (TKTase)
High risk HCPNo
MW (kDa)67.7
pI7.6
UniProt Accession No.G3GUU5 
No. of amino acids623
Triosephosphate isomerase (TPI) No16.48.5G3HNV4150 Read more
Identified HCP (Abbreviation)Triosephosphate isomerase (TPI)
High risk HCPNo
MW (kDa)16.4
pI8.5
UniProt Accession No.G3HNV4
No. of amino acids150
Tubulin alpha-1A chain (TBA1A) No50.14.9P68362451 Read more
Identified HCP (Abbreviation)Tubulin alpha-1A chain (TBA1A)
High risk HCPNo
MW (kDa)50.1
pI4.9
UniProt Accession No.P68362
No. of amino acids451
Tubulin alpha-1C chain (TBA1C) No49.95P68365449 Read more
Identified HCP (Abbreviation)Tubulin alpha-1C chain (TBA1C)
High risk HCPNo
MW (kDa)49.9
pI5
UniProt Accession No.P68365
No. of amino acids449
V-type proton ATPase subunit C 1 (VATC1) No43.97G3I3N5382 Read more
Identified HCP (Abbreviation)V-type proton ATPase subunit C 1 (VATC1)
High risk HCPNo
MW (kDa)43.9
pI7
UniProt Accession No.G3I3N5
No. of amino acids382
Vimentin (VIME) No51.84.9P48670448 Read more
Identified HCP (Abbreviation)Vimentin (VIME)
High risk HCPNo
MW (kDa)51.8
pI4.9
UniProt Accession No.P48670
No. of amino acids448

Advanced Therapy Medicinal Products

Advanced Therapy Medicinal Products support the quest for better and faster development of cell, gene and RNA therapies​ through connecting therapy developers, contract manufacturing, and testing organizations to gain an understanding and respond to the challenges faced by the advanced therapeutics industry.

Development Group

Development Group accelerates and improves the development process of biopharmaceutical medicines for the benefit of the patient. Replacing isolation with collaboration by providing a “safe” space where subject matter experts can work on sharing solutions and best practice on emerging industry trends, implementation of new technologies and common issues whilst ensuring their intellectual property and confidentiality is protected.

Drug Substance

Drug Substance advances excellence in drug substance manufacturing and testing, acting as an industry voice to accelerate technology adoption, implement post-approval change more rapidly and increase confidence in maintaining compliance.

Fill Finish

Fill Finish accelerates development and acceptance of sustainable world-class filling and packaging operations for drug product which meets the future needs of patients. Through innovative solutions resulting from the sharing of expertise, we can overcome common performance challenges and deliver on quality and regulatory compliance.

Information Technology

Information Technology accelerates digital maturity across the ​global pharmaceutical manufacturing industry.

Quality

BioPhorum Quality provides a dedicated, safe space for quality professionals from biomanufacturers and contract organizations to coalesce and enhance effective collaboration to address shared industry challenges.

Regulatory-CMC

Regulatory CMC brings leaders together who have a common goal – to improve access to medicines through innovation in the Regulatory ecosystem. It provides a dedicated space for strategic leadership and a coordinated industry voice, to move from divergence to convergence.

Supply Chain to Patient

Supply Chain to Patient transforms the performance of global pharmaceutical clinical and commercial outbound supply chains through industry collaboration by creating transparent patient-centric, resilient and agile E2E supply chains, that consistently deliver high-quality medicines ensuring an uninterrupted supply to patients.

Supply Partner

Supply Partner is a trusted advisory group and collaboration that is the engine for driving change across the global industry for all things relevant to the inbound supply chain. Addressing the challenges of complexity, resilience, cost, sustainability, and innovation.

Sustainability

Sustainability enables the industry’s transition to a low carbon, circular future – supporting members to improve patient health while respecting the planet. We activate multi-disciplinary teams drawn from our network to deliver environmental sustainability improvements across the value chain.

Technology Strategy

Technology Strategy acts as one voice of the industry to define strategy, accelerate technology transformation and support technology translation and adoption. With a purpose of enabling the future state of flexible, resilient, sustainable end-to-end biomanufacturing.

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An industry perspective on understanding AAV capsid content variants
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EXTERNAL-RESOURCE
An intercompany perspective on practical experiences of predicting, optimizing and analyzing high concentration biologic therapeutic formulations
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The Drug Substance 2.0 Strategic Value Framework
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BioPhorum’s holistic approach to container closure integrity
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Digital Plant Maturity Model 3.0
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Inaugural BioPhorum Quality face to face – get involved
PODCAST
The next step in building regulatory as a capability to enable strategic innovation across the BioPhorum community
PODCAST
Delivering a Cell and Gene Therapy Outbound Supply Chain Pathway for the Industry
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A vision for the biopharmaceutical industry’s inbound supply chain
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A major milestone – a harmonized approach to product carbon footprint data
Technology Roadmap V 2.0
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BioPhorum Technology Roadmapping roadmap vision 2.0

Publications

Documents including best practices, vision statements and peer reviewed papers to help you take value back to your business.

Webinars

Discussing all aspects of the biopharmaceutical industry, from digitization and knowledge management to raw materials, supply chain, sustainability and more. Learn from the perspectives of those at the forefront of the industry.

Podcasts

BioPhorum Connect is our podcast series that keeps you up to date with the latest news and trends in the biopharmaceutical industry. From experienced professionals to thought leaders, these podcasts bring you the insights and perspectives of experts from around the world.

Tools

A variety of resources including design tools and user requirement specifications to help you improve and streamline your business processes.

Benchmarks and Surveys

Research conducted among the BioPhorum membership providing evidence to support change and identify best practice.

Browse all

Access a variety of free tools and papers and other resources designed to provide you with up-to-date information and insights to help you make informed decisions and maximize your success. Access our resources today and start making improving and streamlining your business processes.

WEBINAR
Bioreactivity testing in single-use system biomanufacturing
WEBINAR
Bioreactivity testing in single-use system biomanufacturing
WEBINAR
Bioreactivity testing in single-use system biomanufacturing
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Digital Plant Maturity Model 3.0
WEBINAR
Bioreactivity testing in single-use system biomanufacturing